Microbial metagenome and metatranscriptome sequencing (Illumina and ONT)
The JMF provides library preparation and sequencing services for microbial metagenomes and metatranscriptomes on ONT and Illumina platforms.
Standard Library Preparation workflows include:
- NEBNext UltraExpress FS DNA Library Prep
- NEBNext Ultra II Directional RNA Library Prep in combination with oligo capture or RNAse H based depletion using the:
- Illumina Ribo-Zero Plus rRNA depletion
- a variety of by-request designed rRNA depletion kits ("riboPOOLs") sourced from siTools
- ONT Ligation Sequencing (single-sample libraries)
- ONT Native Barcoding (1-24 sample libraries)
- ONT Rapid Barcoding (up to 96 sample libraries)
Other commercial library preparation workflows are available on request (dedicated consumable orders will be necessary).
For a consultation on the recommended method of choice, with respect to your project goals, project volume and sample type, please contact us directly at info.jmf@univie.ac.at.
Please note:
We perform ONT and Illumina MiSeq sequencing in house, while larger-output volume Illumina projects (NovaSeqX, NovaSeq6000 and NextSeq) are sequenced on equipment hosted by larger local facilities.
