Highly multiplexed gene amplicon sequencing (Illumina)

The JMF provides highly multiplexed gene amplicon sequencing using an in-house developed two-step barcoding system (Pjevac et al., 2021; schematic workflow depicted bellow). This highly cost effective approach is easily expandable to new target genes with minimal time investment in the laboratory. 

For select gene targets, (e.g., 16S rRNA, 18S rRNA, ITS region), quantification via digital PCR can also be performed by the JMF.

Further information

For a detailed protocol of our amplicon sequencing approach, please refer to our publication Pjevac et al. 2021.